Targeted nuclear antisense RNA mimics natural antisense-induced degradation of polyoma virus early RNA.
نویسندگان
چکیده
منابع مشابه
Targeted nuclear antisense RNA mimics natural antisense-induced degradation of polyoma virus early RNA.
We describe a general antisense strategy to inhibit target gene expression. The substitution of a cis-acting ribozyme for a polyadenylylation signal in an antisense expression vector results in the nuclear retention of RNAs and the efficient degradation of their targets. We demonstrate the utility of this system in polyoma virus, where early-strand RNA levels are downregulated in the nucleus by...
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ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 1994
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.91.10.4258